Effects of Feed Additives and Mixed Eimeria Species Infection on Intestinal Microbial Ecology of Broilers

Evaluation of digestive microbial ecology is necessary to understand effects of growth-promoting feed. In the current study, the dynamics of intestinal microbial communities (MC) were examined in broilers fed diets supplemented with a combination of antibiotic (bacitracin methylene disalicylate) and ionophore (Coban 60), and diets containing 1 of 2 essential oil (EO) blends, Crina Poultry (CP) and Crina Alternate (CA). Five treatments were analyzed: 1) unmedicated uninfected control; 2) unmedicated infected control; 3) feed additives monensin (bacitracin methylene disalicylate) + monensin (Coban 60; AI); 4) EO blend CP; and 5) EO blend CA. Additives were mixed into a basal feed mixture, and EO were adjusted to 100 ppm. Chicks were infected by oral gavage at 19 d of age with Eimeria acervulina, Eimeria maxima, and Eimeria tenella. Duodenal, ileal, and cecal samples were taken from 12 birds per treatment just before and 7 d after challenge; 2 samples each were pooled to give a final number of 6 samples total; and all pooled samples were frozen until used for DNA extraction. Denaturing gradient gel electrophoresis was used to examine PCR-amplified fragments of the bacterial 16S ribosomal DNA variable region. Results are presented as percentages of similarity coefficients (SC). Dendrograms of PCR amplicon or band patterns indicated MC differences due to intestinal location, feed additives, and cocci challenge. Essential oil blends CP and CA affected MC in all gut sections. Each EO had different effects over MC, and they differed in most instances from the AI group. The cocci challenge caused drastic MC population shifts in duodenal, ileal, and cecal sections (36.7, 55.4, and 36.2% SC, respectively). Diets supplemented with CP supported higher SC between pre- and postchallenge MC (89.9, 83.3, and 76.4%) than AI (81.8., 57.4, and 60.0%). We concluded that mixed coccidia challenge caused drastic shifts in MC. These EO blends modulated MC better than AI, avoiding drastic shifts after a mixed challenge

Intestinal Microbial Ecology of Broilers Vaccinated and Challenged With Mixed Eimeria Species, and Supplemented with Essential Oil Blends

Intestinal microbiota is an important component in the development of defense mechanisms in the gut mucosa. This project determined the dynamics of intestinal microbial communities (MC) of broilers vaccinated at first day of age with live oocysts of Eimeria species and fed diets supplemented with 2 specific essential oil (EO) blends, Crina Poultry (CP) and Crina Alternate (CA). Five treatments were analyzed: 1) unmedicated-uninfected (UU) control; 2) unmedicated-infected (UI) control; 3) vaccinated with Advent cocci-vaccine and without feed additive (COV) supplements; 4) vaccinated with Advent and supplemented with CP; and 5) vaccinated with Advent and supplemented with CA. The EO blends were added at 100 ppm to the same basal diets. Chicks were gavage-infected at 19 d of age with Eimeria acervulina, Eimeria maxima, and Eimeria tenella. Duodenal, ileal, and cecal samples were taken from 12 birds per treatment just before the infection and 7 d after the challenge, pooled in 6 samples, and frozen. Denaturing gradient gel electrophoresis was used to examine PCR-amplified fragments of the bacterial 16S ribosomal DNA variable region. Results are presented as percentages of similarity coefficients (SC). Dendrograms of amplicon patterns indicated MC differences due to intestinal location, feed additives, and cocci infection. The EO blends CP and CA did affect MC in all gut sections. The cocci-infection caused drastic MC population shifts in duodenal, ileal, and cecal sections (36.7, 55.4, and 36.2% SC, respectively). The CP-supplemented birds had higher SC between pre- and postchallenge MC in duodenal and ileal (73.3, 81.8%) than COV (66.4, 66.5%). However, COV broilers had the smallest changes in cecal MC after infection (79.5% SC). We concluded that cocci-vaccination causes small changes in intestinal MC, but challenge causes drastic shifts. The EO blend supplementation modulates MC in cocci-vaccinated broilers, avoiding drastic shifts after a mixed coccidia infection. Correlations between MC dynamics and host responses are discussed

Responses of Coccidia-Vaccinated Broilers to Essential Oil Blends Supplementation up to Forty-Nine Days of Age

Coccidiosis control may become a greater problem as the use of growth-promoting antibiotics (GPA) and ionophores declines. Vaccination with live oocysts may turn into a popular alternative to the use of coccidiostats in broilers, although cocci vaccination is frequently linked to temporary lower performance in young flocks. This experiment evaluates the dietary supplementation of 2 specific essential oil (EO) blends (Crina Poultry and Crina Alternate), either as alternatives to GPA and ionophores (BMD + Coban) or as feed additives that help to improve the performance of cocci-vaccinated broilers. Live performance and lesion scores were observed. These 2 specific EO blends differ in their efficacy to promote growth. Chickens that were not cocci vaccinated and were fed Crina Poultry had better feed conversion ratio (FCR) than the unmedicated control treatment in the starter period. The same EO improved FCR in cocci-vaccinated birds in the finisher period in comparison to the negative control group, but those responses were not significantly different from other treatments or significant at 49 d of age. No significant differences were observed in lesion scores at 37 d. Diets supplemented with a GPA-ionophore combination consistently supported the best BW gain and FCR in each period and the entire grow-out period. No significant beneficial or deleterious effects on live performance were observed due to these specific EO blends in cocci-vaccinated broilers

Failure pressure and fatigue analysis of the API 12F shop welded, flat bottom tanks

This study investigates the failure pressure on the API 12F shop welded steel tanks and performs a fatigue evaluation to estimate the permissible number of pressure cycles for these equipment. Four different analyses were carried out on more than 350 finite element models to determine various failure pressure modes of these storage tanks. An elastic analysis considering potential buckling modes was developed to determine the yielding pressure of the tanks. The redistribution of stresses due to inelastic deformations and plastic collapse were evaluated through an elastic-plastic stress analysis considering the plastic hardening of the material. A wind load analysis was performed to evaluate the stress levels at all regions of the tank and estimate the uplift deformations. Moreover, the increase of the design pressure was investigated regarding the stress levels and bottom uplift. Additionally, an elastic stress analysis following the ASME Boiler & Pressure Vessel Code Section VIII, Division 2, Design-by-Analysis rules was implemented to determine the fatigue life of the storage tanks. This research provides engineering calculations to evaluate the current design of the API 12F tanks and the design internal pressures guaranteeing a safe performance of the equipment

Effect of Electrolyte Balance in Low-Protein Diets on Broiler Performance and Tibial Dyschondroplasia Incidence

A proper dietary electrolyte balance (DEB) is essential to ensure an optimum acid-base equilibrium and broiler performance. In low-CP diets, this balance can be affected by reduction of soybean meal and inclusion of high levels of synthetic amino acids. Although, some studies have related low-protein diets supplemented with amino acids and DEB, these relations are not well explained, because some research demonstrates confusion about the deficiency and balance of nutrients. The objective of these experiments was to evaluate the DEB effects of diets with low levels of protein supplemented with amino acids on broiler performance and bone development. Results indicated that DEB and CP content influenced broiler chick performance in the starter and growing periods. There was no significant effect due to the interaction between DEB and CP content for tibial dyschondroplasia incidence (TD) or in bone breaking resistance during the growing period of either experiment. The incidence of TD was reduced with 253 mEq/kg DEB in the starter period

Soil mineral N dynamics beneath mixtures of leaves from legume and fruit trees in Central Amazonian multi-strata agroforests.

Dinâmica do nitrogênio mineral no solo em misturas de folhas de leguminosas arbóreas e de fruteiras em sistemas agroflorestais multiestratificados na Amazônia Central

Enzymes as Feed Additive to Aid in Responses Against Eimeria Species in Coccidia-Vaccinated Broilers Fed Corn-Soybean Meal Diets with Different Protein Levels

This research aimed to evaluate the effects of adding a combination of exogenous enzymes to starter diets varying in protein content and fed to broilers vaccinated at day of hatch with live oocysts and then challenged with mixed Eimeria spp. Five hundred four 1-d-old male Cobb-500 chickens were distributed in 72 cages. The design consisted of 12 treatments. Three anticoccidial control programs [ionophore (IO), coccidian vaccine (COV), and coccidia-vaccine + enzymes (COV + EC)] were evaluated under 3 CP levels (19, 21, and 23%), and 3 unmedicated-uninfected (UU) negative controls were included for each one of the protein levels. All chickens except those in unmedicated-uninfected negative controls were infected at 17 d of age with a mixed oral inoculum of Eimeria acervulina, Eimeria maxima, and Eimeria tenella. Live performance, lesion scores, oocyst counts, and samples for gut microflora profiles were evaluated 7 d postinfection. Ileal digestibility of amino acids (IDAA) was determined 8 d postinfection. Microbial communities (MC) were analyzed by G + C%, microbial numbers were counted by flow cytometry, and IgA concentrations were measured by ELISA. The lowest CP diets had poorer (P ≤ 0.001) BW gain and feed conversion ratio in the preinfection period. Coccidia-vaccinated broilers had lower performance than the ones fed ionophore diets during pre- and postchallenge periods. Intestinal lesion scores were affected (P ≤ 0.05) by anticoccidial control programs, but responses changed according to gut section. Feed additives or vaccination had no effect (P ≥ 0.05) on IDAA, and diets with 23% CP had the lowest (P ≤ 0.001) IDAA. Coccidial infection had no effect on MC numbers in the ileum but reduced MC numbers in ceca and suppressed ileal IgA production. The COV + EC treatment modulated MC during mixed coccidiosis infection but did not significantly improve chicken performance. Results indicated that feed enzymes may be used to modulate the gut microflora of cocci-vaccinated broiler chickens

Carbon and nutrient accumulation in secondary forests regenerating on pastures in central Amazonia

Copyright by the Ecological Society of America ©2004. Feldpausch, TR, Rondon, MA, Fernandes, ECM, Riha, SJ and Wandelli, E (2004) Carbon and nutrient accumulation in secondary forests regenerating on pastures in central Amazonia. Ecological Applications, 14 (4). S164 - S176 . ISSN 1051-0761 http://dx.doi.org/10.1890/01-6015Over the past three decades, large expanses of forest in the Amazon Basin were converted to pasture, many of which later degraded to woody fallows and were abandoned. While the majority of tropical secondary forest (SF) studies have examined post-deforestation or post-agricultural succession, we examined post-pasture forest recovery in 10 forests ranging in age from 0 to 14 years since abandonment. We measured aboveground biomass and soil nutrients to 45 cm depth and computed total site carbon (C) and nutrient stocks to gain an understanding of the dynamics of nutrient and C buildup in regenerating SF in central Amazonia. Aboveground biomass accrual was rapid, 11.0 Mg·ha−1·yr−1, in the young SFs. Within 12–14 yr, they accumulated up to 128.1 Mg/ha of dry aboveground biomass, equivalent to 25–50% of primary forest biomass in the region. Wood nitrogen (N) and phosphorus (P) concentrations decreased with forest age. Aboveground P and calcium (Ca) stocks accumulated at a rate of 1.2 and 29.4 kg·ha−1·yr−1; extractable soil P stocks declined as forest age increased. Although soil stocks of exchangeable Ca (207.0 ± 23.7 kg/ha) and extractable P (8.3 ± 1.5 kg/ha) were low in the first 45 cm, both were rapidly translocated from soil to plant pools. Soil N stocks increased with forest age, probably due to N fixation, atmospheric deposition, and/or subsoil mining. Total soil C storage to 45 cm depth ranged between 42 and 84 Mg/ha, with the first 15 cm storing 40–45% of the total. Total C accrual (7.04 Mg C·ha−1·yr−1) in both aboveground and soil pools was similar or higher than values reported in other studies. Tropical SFs regrowing on lightly to moderately used pasture rapidly sequester C and rebuild total nutrient capital following pasture abandonment. Translocation of some nutrients from deep soil (>45 cm depth) may be important to sustaining productivity and continuing biomass accumulation in these forests. The soil pool represents the greatest potential for long-term C gains; however, soil nutrient deficits may limit future productivity

Requirements of Sodium and Chloride by Leghorn Laying Hens

Sodium and Cl are low-cost nutrients with great influence on feed conversion ratio (FCR), eggshell quality, and excreta moisture. Actual values of dietary requirements of these minerals for commercial laying hens are not well defined. These requirements were reevaluated in a factorial experiment using corn-soybean meal basal diets. No significant influence of Na and Cl levels was observed on egg production (%), egg weight (g), or feed intake (g/d), but levels of these minerals had variable effects on FCR, eggshell quality, and excreta moisture. The optimum requirement of Na changed according to the variable evaluated and level of Cl used

La enseñanza de las ciencias mediante proyectos didácticos en la Universidad de los Andes - Venezuela

Los paradigmas de la pedagogía científica incorporan estrategias activas, pero, en el trabajo de aula sigue prevaleciendo el modelo pasivo. En contraposición a esto, el presente ensayo describe la experiencia que hemos acumulado desde 1999 con el desarrollo de una metodología denominada Proyectos Didácticos para la Enseñanza de la Ciencia que tiene por finalidad principal aportar diferentes propuestas metodológicas para abordar el trabajo de aula y fuera de ésta, para el caso especial de la enseñanza de las ciencias. Esta estrategia implica la participación activa del estudiante diseñando propuestas prácticas o juegos para el desarrollo de diversos contenidos típicos de la ciencia en el contexto de la formación inicial de docentes en la Universidad de Los Andes